Journal article
Functional interaction between angiotensin ii receptor type 1 and chemokine (C-C motif) receptor 2 with implications for chronic kidney disease
MA Ayoub, Y Zhang, RS Kelly, HB See, EKM Johnstone, EA McCall, JH Williams, DJ Kelly, KDG Pfleger
Plos One | PUBLIC LIBRARY SCIENCE | Published : 2015
Abstract
Understanding functional interactions between G protein-coupled receptors is of great physiological and pathophysiological importance. Heteromerization provides one important potential mechanism for such interaction between different signalling pathways via macromolecular complex formation. Previous studies suggested a functional interplay between angiotensin II receptor type 1 (AT1) and Chemokine (C-C motif) Receptor 2 (CCR2). However the molecular mechanisms are not understood.We investigated AT1-CCR2 functional interaction in vitro using bioluminescence resonance energy transfer in HEK293 cells and in vivo using subtotal-nephrectomized rats as a well-established model for chronic kidney d..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
This work was partly funded by Dimerix Bioscience Limited (www.dimerix.com), who was itself supported by the Australian Government's Commercialisation Australia scheme. Subsequently, this work was funded in part by the Australian Research Council (ARC; www.arc.gov.au) via Discovery Project Grant DP120101297 (KDGP, DJK and MAA). KDGP was an ARC Future Fellow (FT100100271) and is now a National Health and Medical Research Council of Australia (NHMRC; www.nhmrc.gov.au) RD Wright Fellow (1085842). EKMJ was funded by the Richard Walter Gibbon Medical Research Scholarship from The University of Western Australia (www.uwa.edu.au). DJK was funded by a NHMRC Senior Research Fellowship (566867). Other than EAM, JHW and KDGP, the funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.